UV/Visible Spectrophotometer
Ultraviolet Analysis
Equipment
Ultraviolet Double-Beam
Spectrophotometer, Perkin-Elmer Lambda 3B with integrating
sphere accessory.
The UV method is applied to organic analysis. Molecules with two or more
isolated chromophores (absorbing groups) absorb light of nearly the same
wavelength as does a molecule containing only a single chromophore of a
particular type. The intensity of the absorption is proportional to the
number of that type of chromophore present in the molecule.
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Characterization
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